CD209a Antigen antibody

Catalog No. ABIN2191829

Product Details anti-CD209a Antigen Antibody

Target: CD209a Antigen (CD209A)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD209a Antigen antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Endotoxin Level: Low endotoxin level

Clone: ER-TR9

Application Details

Application Notes: For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. 1

Restrictions: For Research Use only

Handling

Buffer: 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-CD209a Antigen antibody (ABIN2191829)

Takagi, Numazaki, Kajiwara, Abe, Ishii, Kato, Kojima: "Cooperation of specific ICAM-3 grabbing nonintegrin-related 1 (SIGNR1) and complement receptor type 3 (CR3) in the uptake of oligomannose-coated liposomes by macrophages." in: Glycobiology, Vol. 19, Issue 3, pp. 258-66, (2009) (PubMed).

Nagaoka, Takahara, Tanaka, Yoshida, Steinman, Saitoh, Akashi-Takamura, Miyake, Kang, Park, Inaba: "Association of SIGNR1 with TLR4-MD-2 enhances signal transduction by recognition of LPS in gram-negative bacteria." in: International immunology, Vol. 17, Issue 7, pp. 827-36, (2005) (PubMed).

Kang, Kim, Bruening, Pack, Charalambous, Pritsker, Moran, Loeffler, Steinman, Park: "The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 1, pp. 215-20, (2004) (PubMed).

Taylor, Brown, Herre, Williams, Willment, Gordon: "The role of SIGNR1 and the beta-glucan receptor (dectin-1) in the nonopsonic recognition of yeast by specific macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 2, pp. 1157-62, (2004) (PubMed).

Kretschmer, Jungebloud, Stopkowicz, Kleinke, Hoffmann, Weiss: "The selection of marginal zone B cells differs from that of B-1a cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 12, pp. 6495-501, (2003) (PubMed).

Target Details for CD209a Antigen

Target: CD209a Antigen (CD209A)

Alternative Name: Sign-r1 (CD209A Products)

Synonyms: CD209 antibody, CDSIGN antibody, CIRE antibody, DC-SIGN antibody, DC-SIGN1 antibody, Dcsign antibody, SIGN-R1 antibody, SIGNR5 antibody, CD209a antigen antibody, CD209a molecule antibody, Cd209a antibody

Background: The monoclonal antibody ER-TR9 recognizes murine SIGN-related 1 (SIGN-R1). Mouse SIGN-R1, a homolog of human DC-SIGN, is a 37 kDa type II transmembrane protein containing a single, C- terminal C-type lectin domain. SIGN-R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in the peritoneal cavity of some mouse strains. ER-TR9 does not react with macrophages in other regions of the spleen, such as CD169+ marginal metallophils and F4/80+ red pulp macrophages. In the spleen, the MZM function in trapping and clearance of blood- borne microbial antigens. SIGN-R1 mediates the uptake of encapsulated microbes , particularly through the recognition of microbial polysaccharides. Uptake of FITC-labeled dextran by macrophages can be blocked both in vivo and in vitro by the monoclonal antibody ER-TR9. Therefore, the monoclonal antibody ER-TR9 can be used to study the uptake of polysaccharides by macrophages. Aliases CD209b, specific intracellular adhesion molecule-3 grabbing nonintegrin homolog-related 1 Immunogen Mouse thymic stromal cells