C5AR
Reactivity: Mouse
FACS, Func, IF
Host: Rat
Monoclonal
20-70
unconjugated
Application Notes
For immunohistology, Western blotting and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date
12 months
Soruri, Kim, Kiafard, Zwirner: "Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody." in: Immunology letters, Vol. 88, Issue 1, pp. 47-52, (2003) (PubMed).
The monoclonal antibody 10/92 recognizes the mouse receptor C5a (C5aR, CD88). The mouse anaphylatoxin C5a is a 74-residu glycopolypeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a could be demonstrated, rendering this molecule an important mediator of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP- binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by neutrophils, monocytes/macrophages, dendritic cells, astrocytes and microglia. In contrast, C5a receptor is not detectable on resting or stimulated murine T and B lymphocytes. The monoclonal antibody 10/92 does not inhibit the binding of C5a to its receptor. Aliases C5aR1, CD88 Immunogen RBL-2H3 transfectants expressing murine C5aR