JAM3 antibody

Catalog No. ABIN2191807

Product Details anti-JAM3 Antibody

Target: JAM3 (Junctional Adhesion Molecule 3 (JAM3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This JAM3 antibody is un-conjugated

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA), Detection (D), Inhibition Assay (InhA)

Sterility: 0.2 μm filtered

Clone: CRAM-18 F26

Application Details

Application Notes: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity, dilutions have to be made according to the amounts of JAM-C to be inhibited.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-JAM3 antibody (ABIN2191807)

Johnson-Léger, Aurrand-Lions, Beltraminelli, Fasel, Imhof: "Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration." in: Blood, Vol. 100, Issue 7, pp. 2479-86, (2002) (PubMed).

Target Details for JAM3

Target: JAM3 (Junctional Adhesion Molecule 3 (JAM3))

Alternative Name: Junctional Adhesion Molecule-C (JAM3 Products)

Synonyms: JAM3 antibody, jam3 antibody, sr:nyz155 antibody, wu:fb30h11 antibody, wu:fc08c06 antibody, wu:fc13e04 antibody, wu:fc25g11 antibody, jamc.2 antibody, 1110002N23Rik antibody, JAM-3 antibody, JAM-C antibody, Jcam3 antibody, JAM-2 antibody, JAMC antibody, junctional adhesion molecule 3 antibody, junctional adhesion molecule 3b antibody, junctional adhesion molecule 3a antibody, junction adhesion molecule 3 antibody, JAM3 antibody, jam3b antibody, jam3a antibody, Jam3 antibody

Background: The monoclonal antibody CRAM-18 F26 recognizes junctional adhesion molecule-C (JAM-C) also known as JAM-2, a 45 kD cell adhesion molecule (CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. Monoclonal antibody CRAM-18 F26 also reacts with human JAM-C. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.