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HCST antibody (AA 19-48) (HRP)

HCST Reactivity: Human, Rat WB, ELISA, IHC (fro), IHC (p) Host: Rabbit Polyclonal HRP
Catalog No. ABIN2175302
  • Target See all HCST Antibodies
    HCST (Hematopoietic Cell Signal Transducer (HCST))
    Binding Specificity
    AA 19-48
    Reactivity
    Human, Rat
    Host
    • 15
    Rabbit
    Clonality
    • 15
    Polyclonal
    Conjugate
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HCST antibody is conjugated to HRP
    Application
    • 15
    • 14
    • 13
    • 13
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Cross-Reactivity
    Human, Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human HCST
    Isotype
    IgG
    Top Product
    Discover our top product HCST Primary Antibody
  • Application Notes
    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    HCST (Hematopoietic Cell Signal Transducer (HCST))
    Alternative Name
    HCST (HCST Products)
    Synonyms
    DAP10 antibody, KAP10 antibody, PIK3AP antibody, Dap10 antibody, HCST antibody, dap10 antibody, dap10-A antibody, hcst antibody, hcsta antibody, kap10 antibody, pik3ap antibody, hematopoietic cell signal transducer antibody, hematopoietic cell signal transducer L homeolog antibody, HCST antibody, Hcst antibody, hcst antibody, hcst.L antibody
    Background

    Synonyms: DAP10, KAP10, PIK3AP, Hematopoietic cell signal transducer, DNAX-activation protein 10, Membrane protein DAP10, Transmembrane adapter protein KAP10, HCST, UNQ587/PRO1157

    Background: Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells, this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs), these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells, indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells.

    Gene ID
    10870
    UniProt
    Q9UBK5
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