ELISA: 0.5-2 μg/mL, Inhibition: 0.05-0.1 μg/mL, WB: 0.1-0.2 μg/mL Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in sterile water to a concentration no less than 0.2 mg/mL.
Concentration
Lot specific
Buffer
Lyophilized from PBS, pH 7.2.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.
VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase (flt-1), kDaR gene product (the Mouse homolog of kDaR is the flk-1 gene product) and the flt4 gene product.