RAD54L antibody (C-Term, N-Term)

Catalog No. ABIN214236

Product Details anti-RAD54L Antibody

Target: RAD54L (RAD54-Like (RAD54L))

Binding Specificity: AA 1-300, C-Term, N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD54L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Peptides from N and C termini of RAD54L

Purification: Protein G purified

Immunogen: Combination of N & C peptide, corresponding to a range between amino acids 1-300 and 450-741

Type of Immunogen: Synthetic peptide

Clone: 60-1

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2. No preservatives added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RAD54L

Target: RAD54L (RAD54-Like (RAD54L))

Alternative Name: RAD54L (RAD54L Products)

Background: Name/Gene ID: RAD54L

Synonyms: RAD54L, HR54, HRAD54, RAD54 (S.cerevisiae)-like, RAD54-like (S. cerevisiae), RAD54, RAD54 homolog, RAD54A, HHR54

Gene ID: 8438

UniProt: P46100

Pathways: DNA Damage Repair