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PUMA antibody (C-Term)

BBC3 Reactivity: Human, Mouse WB, IHC, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN213906
  • Target See all PUMA (BBC3) Antibodies
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Binding Specificity
    • 16
    • 12
    • 9
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 91
    • 40
    • 37
    • 16
    Human, Mouse
    Host
    • 88
    • 6
    • 1
    Rabbit
    Clonality
    • 80
    • 15
    Polyclonal
    Conjugate
    • 47
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PUMA antibody is un-conjugated
    Application
    • 76
    • 34
    • 28
    • 21
    • 15
    • 14
    • 13
    • 12
    • 9
    • 6
    • 5
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.
    Purification
    Immunoaffinity purified
    Isotype
    IgG
    Top Product
    Discover our top product BBC3 Primary Antibody
  • Application Notes
    Approved: ICC, IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    avoid freeze thaw cycles. Store undiluted.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Alternative Name
    PUMA (BBC3 Products)
    Synonyms
    BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
    Background
    Name/Gene ID: BBC3
    Family: Apoptosis

    Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA
    Gene ID
    27113
    UniProt
    Q9BXH1
    Pathways
    p53 Signaling, Positive Regulation of Endopeptidase Activity
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