USP4 antibody (Internal Region)

Catalog No. ABIN213585

Product Details anti-USP4 Antibody

Target: USP4 (Ubiquitin Specific Peptidase 4 (USP4))

Binding Specificity: Internal Region

Reactivity: Human, Rabbit, Pig, Dog, Horse, Monkey, Cow, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human USP4. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except USP15 (67 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig (100%) Hamster, Opossum (94%) Mouse, Rat, Turkey, Platypus (89%) Chicken, Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from internal region of human USP4. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig (100%), Hamster, Opossum (94%), Mouse, Rat, Turkey, Platypus (89%), Chicken, Xenopus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for USP4

Target: USP4 (Ubiquitin Specific Peptidase 4 (USP4))

Alternative Name: UNP / USP4 (USP4 Products)

Synonyms: UNP antibody, Unph antibody, F730026I20Rik antibody, Unp antibody, mKIAA4155 antibody, ubiquitin specific peptidase 4 antibody, ubiquitin specific peptidase 4 (proto-oncogene) antibody, USP4 antibody, Usp4 antibody

Background: Name/Gene ID: USP4
Subfamily: Cysteine C19
Family: Protease

Synonyms: USP4, Deubiquitinating enzyme 4, Unph, Ubiquitin thiolesterase 4, Ubiquitin thioesterase 4, Ubiquitin-specific protease 4, UNP

Gene ID: 7375