UCHL3 antibody (Internal Region)

Catalog No. ABIN213583

Product Details anti-UCHL3 Antibody

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Dog, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human UCHL3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Dog, Elephant, Opossum, Turkey, Chicken, Lizard (100%) Orangutan, Monkey, Bovine, Horse, Rabbit, Pig, Salmon (94%) Pufferfish, Zebrafish, Stickleback, Water flea (89%) Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from internal region of human UCHL3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Dog, Elephant, Opossum, Turkey, Chicken, Lizard (100%), Orangutan, Monkey, Bovine, Horse, Rabbit, Pig, Salmon (94%), Pufferfish, Zebrafish, Stickleback, Water flea (89%), Xenopus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: UCHL3 (Uchl3 Products)

Background: Name/Gene ID: UCHL3
Subfamily: Cysteine C12
Family: Protease

Synonyms: UCHL3, Ubiquitin thiolesterase, Ubiquitin thioesterase L3, UCH-L3

Gene ID: 7347

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation