GPR149 antibody (Cytoplasmic Domain)

Catalog No. ABIN213495

Product Details anti-GPR149 Antibody

Target: GPR149 (G Protein-Coupled Receptor 149 (GPR149))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR149 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GPR149. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Elephant (94%) Monkey, Marmoset (88%) Bat (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human GPR149. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Elephant (94%), Monkey, Marmoset (88%), Bat (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR149

Target: GPR149 (G Protein-Coupled Receptor 149 (GPR149))

Alternative Name: GPR149 / PGR10 (GPR149 Products)

Synonyms: GPR149 antibody, IEDA antibody, PGR10 antibody, Ieda antibody, 9630018L10Rik antibody, R35 antibody, G protein-coupled receptor 149 antibody, probable G-protein coupled receptor 149 antibody, GPR149 antibody, LOC100219033 antibody, gpr149 antibody, Gpr149 antibody

Background: Name/Gene ID: GPR149
Subfamily: Orphan-U
Family: GPCR

Synonyms: GPR149, PGR10, G protein-coupled receptor 149, IEDA

Gene ID: 344758