EPH Receptor A2 antibody
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- Target See all EPH Receptor A2 (EPHA2) Antibodies
- EPH Receptor A2 (EPHA2)
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Reactivity
- Human, Mouse, Rat, Dog, Cow
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EPH Receptor A2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)
- Specificity
- Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.
- Purification
- Protein G purified
- Immunogen
- Native protein isolated by purification of phosphotyrosine-containing proteins.
- Isotype
- IgG1
- Top Product
- Discover our top product EPHA2 Primary Antibody
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- Application Notes
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Approved: Func, ICC, IP, WB
Usage: Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 μg of a human breast epithelial cell line which had been lysed in TBS containing 1 % Triton X-100. Use 1-4 μg per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7 % formaldehyde solution and permeabilized with 0.5 % Triton X-100 in TBS. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- EPH Receptor A2 (EPHA2)
- Alternative Name
- EPHA2 / EPH Receptor A2 (EPHA2 Products)
- Synonyms
- ARCC2 antibody, CTPA antibody, CTPP1 antibody, CTRCT6 antibody, ECK antibody, epha2 antibody, MGC89505 antibody, AW545284 antibody, Eck antibody, Myk2 antibody, Sek-2 antibody, Sek2 antibody, id:ibd5152 antibody, rtk6 antibody, G42 antibody, XE10 antibody, epha2-A antibody, EPH receptor A2 antibody, Eph receptor A2 antibody, epoxide hydrolase antibody, eph receptor A2 a antibody, EPH receptor A2 L homeolog antibody, EPHA2 antibody, Epha2 antibody, epha2 antibody, ephA2 antibody, epha2a antibody, epha2.L antibody
- Background
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Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase
Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1 - Gene ID
- 1969
- UniProt
- P29317
- Pathways
- RTK Signaling
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