FYB antibody (AA 755-768)
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- Target See all FYB Antibodies
- FYB (FYN-Binding Protein (FYB))
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Binding Specificity
- AA 755-768
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Reactivity
- Human, Mouse, Rat, Dog, Horse, Cow, Pig, Monkey, Bat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FYB antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- Recognizes ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide. Species sequence homology: Human and rat.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Pig (100%) Platypus (93%).
- Purification
- Protein A purified
- Immunogen
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Synthetic peptide corresponding to amino acids 755-768 (DNDGEIYDDIADGC) of mouse Fyn binding protein (ADAP/SLAP-130/Fyb) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Pig (100%), Platypus (93%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG
- Top Product
- Discover our top product FYB Primary Antibody
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- Application Notes
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Approved: IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated ADAP/SLAP-130/Fyb from 500 μg of RAW. 264.7 macrophage RIPA lysate.
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- FYB (FYN-Binding Protein (FYB))
- Alternative Name
- ADAP / FYB (FYB Products)
- Synonyms
- fyb antibody, ADAP antibody, PRO0823 antibody, SLAP-130 antibody, B630013F22Rik antibody, FYB-120/130 antibody, Slap130 antibody, p120/130 antibody, p120/p130 antibody, RGD1563421 antibody, FYN-binding protein antibody, FYN binding protein 1 antibody, FYN binding protein antibody, fyb antibody, FYB1 antibody, Fyb antibody, Fyb1 antibody
- Background
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Name/Gene ID: FYB
Synonyms: FYB, ADAP, FYN-binding protein, FYN-T-binding protein, FYB-120/130, FYN binding protein, p120/p130, PRO0823, SLAP130, SLAP-130 - Gene ID
- 2533
- UniProt
- O15117
- Pathways
- TCR Signaling, Protein targeting to Nucleus
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