RFXANK antibody

Catalog No. ABIN204403

Product Details anti-RFXANK Antibody

Target: RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFXANK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for s all splice forms of Ankyrin G containing a spectrin-binding domain. Does not cross-react with related ankyrin isoforms. Species cross-reactivity: Human, mouse, rabbit, rat, canine.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. ELISA (native): 0.1-1.0 μg/mL. Detects a band between ~200-300kD. Western Blot: 1-3 μg/mL. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3 M sucrose to incubation buffer. Immunofluorescence (frozen sections): 5-10 μg/mL. Sensitive to fixation conditions. Recommend 1 % paraformaldehyde for perfusion and 4 hrs of post fixation, after perfusion. For sciatic nerve, recommend 2 % paraformaldehyde for 2 hrs at 4?C. Immunoprecipitation (native): 5 μg/ IP.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for RFXANK

Target: RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))

Alternative Name: RFXANK (RFXANK Products)

Synonyms: ANKRA1 antibody, BLS antibody, F14150_1 antibody, RFX-B antibody, Tvl1 antibody, regulatory factor X associated ankyrin containing protein antibody, regulatory factor X-associated ankyrin-containing protein antibody, RFXANK antibody, Rfxank antibody

Background: Name/Gene ID: RFXANK

Synonyms: RFXANK, ANKRA1, BLS, DNA-binding protein RFXANK, F14150_1, RFXB, RFX-B, Regulatory factor X subunit B, RFX-Bdelta4

Gene ID: 8625

UniProt: O14593