GAPDH antibody

Catalog No. ABIN2017607

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Pig, Cow, Goat, Fish

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: GAPDH Antibody, mAb, Mouse detects endogenous levels of human, pig, cow, goat, and fish GAPDH.

Cross-Reactivity (Details): This product has been not tested with other species yet.

Characteristics: Fusion Partner: Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Purification: Protein A affinity column

Immunogen: Human GAPDH protein from erythrocytes

Clone: 3B1E9

Isotype: IgG2a kappa

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 μg/mL
Western blot: 0.1-1.0 μg/mL.
Other Application: User optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: Lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-GAPDH antibody (ABIN2017607)

Chen, Caporizzo, Bedi, Vite, Bogush, Robison, Heffler, Salomon, Kelly, Babu, Morley, Margulies, Prosser: "Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure." in: Nature medicine, Vol. 24, Issue 8, pp. 1225-1233, (2019) (PubMed).

Ju, Gao, Hou, Wang, Zhang, Sun, Zhang, Xu, Shi, Hu, Zhang: "Neuroprotective effect of chondroitin sulfate on SH‑SY5Y cells overexpressing wild‑type or A53T mutant α‑synuclein." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8721-8728, (2018) (PubMed).

Wang, Mir: "Andes virus nucleocapsid protein interrupts protein kinase R dimerization to counteract host interference in viral protein synthesis." in: Journal of virology, Vol. 89, Issue 3, pp. 1628-39, (2015) (PubMed).

Yang, Li, Liu, He, Yuan, Li, Cao, Kim: "Mx1-cre mediated Rgs12 conditional knockout mice exhibit increased bone mass phenotype." in: Genesis (New York, N.Y. : 2000), Vol. 51, Issue 3, pp. 201-9, (2013) (PubMed).

Cassel, Reitz: "Ubiquilin-2 (UBQLN2) binds with high affinity to the C-terminal region of TDP-43 and modulates TDP-43 levels in H4 cells: characterization of inhibition by nucleic acids and 4-aminoquinolines." in: Biochimica et biophysica acta, Vol. 1834, Issue 6, pp. 964-71, (2013) (PubMed).

Martins, Collado-Romero, Martínez-Gomáriz, Carvajal, Gil, Lucena, Moreno, Garrido: "Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection." in: Journal of proteomics, Vol. 75, Issue 14, pp. 4457-70, (2012) (PubMed).

Cassel, McDonnell, Velvadapu, Andrianov, Reitz: "Characterization of a series of 4-aminoquinolines that stimulate caspase-7 mediated cleavage of TDP-43 and inhibit its function." in: Biochimie, Vol. 94, Issue 9, pp. 1974-81, (2012) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimers and Huntingtons disease. GAPDH Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with human GAPDH protein from erythrocytes.

Molecular Weight: Predicted Band Size: 36 KD
Observed Band Size: 36 KD