Plakophilin 3 antibody

Catalog No. ABIN201418

Product Details anti-Plakophilin 3 Antibody

Target: Plakophilin 3 (PKP3)

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Plakophilin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Reacts with the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.

Purification: Protein A purified

Immunogen: Synthetic peptide from Armadillo domain of human Plakophilin-3.

Type of Immunogen: Synthetic peptide

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 10 μg/IP reaction. Immunohistochemistry: 10 μg/mL. Immunofluorescence: 1-3 μg/mL. Western Blot: 1-3 μg/mL. Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2 % Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Plakophilin 3

Target: Plakophilin 3 (PKP3)

Alternative Name: PKP3 / Plakophilin 3 (PKP3 Products)

Synonyms: wu:fa97f09 antibody, wu:fk69a12 antibody, zgc:136656 antibody, PKP3 antibody, 2310056L12Rik antibody, plakophilin 3a antibody, plakophilin 3 antibody, plakophilin-3 antibody, plakophilin 3 L homeolog antibody, pkp3a antibody, PKP3 antibody, LOC450921 antibody, pkp3.L antibody, Pkp3 antibody

Background: Name/Gene ID: PKP3

Synonyms: PKP3, Plakophilin 3, Plakophilin-3, Plakophilin 3b

Gene ID: 11187

UniProt: Q9Y446