Laminin antibody

Catalog No. ABIN201206

Product Details anti-Laminin Antibody

Target: Laminin (LN)

Reactivity: Human, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Laminin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: Recognizes human laminin. Typically less than 1 % cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Recognizes most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to regions of adherence on laminin.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:4000), IHC, IHC-P, IP, WB (1:250 - 1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. ELISA: 1:2000-1:4000. Western Blot: 1:250-1:1000. Immunohistochemistry (paraffin embedded sections): 1:25-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 125 mM sodium borate, 75 mM sodium chloride, 5 mM EDTA,  pH 8.0, 0.01 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Laminin

Target: Laminin (LN)

Alternative Name: Laminin (LN Products)

UniProt: P25391