HSPG2 antibody (Domain 3)

Catalog No. ABIN199273

Product Details anti-HSPG2 Antibody

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Binding Specificity: Domain 3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes domain III of human perlecan. Domain III shares homology with the short arm of laminin A and B chains.

Purification: Protein A purified

Immunogen: Recombinant Perlecan domain III.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL. Immunohistochemistry (Frozen or FFPE): 5-10 μg/mL. Note: Formalin-fixed, paraffin sections require pretreatment with 0.1 % protease for 30 minutes at RT prior to the blocking step. Positive control: Human breast, colon, liver, lymph nodes, pancreas and prostate tissues.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Alternative Name: HSPG2 / Perlecan (HSPG2 Products)

Background: Name/Gene ID: HSPG2

Synonyms: HSPG2, Endorepellin (domain V region), HSPG, Heparan sulfate proteoglycan 2, Perlecan, Perlecan proteoglycan, SJA, SJS1, PRCAN, SJS

Gene ID: 3339

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism