VASP antibody (pSer239)

Catalog No. ABIN198238

Product Details anti-VASP Antibody

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Binding Specificity: AA 236-242, pSer239

Reactivity: Human, Mouse, Rat, Monkey, Dog, Cow, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: Recognizes human phosphorylated VASP, Mr 50kD. Species cross-reactivity: mouse.

Purification: Gel filtration

Immunogen: KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE).

Type of Immunogen: Synthetic phospho-peptide

Clone: 16C2

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.05 μg/mL), Flo (2.4 μg/mL), ICC, IP, WB

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, FACS and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: An independent laboratory has reported that 3 μg/mL antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton-X. FACS: 2.4 μg/mL using fixed and permeabilized human platelets, as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. ELISA: 0.05 μg/mL, as reported by an independent laboratory.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL ddH2O

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from PBS, 0.09 % sodium azide, PEG, sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP (VASP Products)

Background: Name/Gene ID: VASP
Family: Ena-VASP

Synonyms: VASP

Gene ID: 7408

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation