CD101 antibody (PE)

Catalog No. ABIN1981894

Product Details anti-CD101 Antibody (PE)

Target: CD101

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD101 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody BB27 recognizes an extracellular epitope of CD101, a 140 kDa disulfide-bonded homodimeric protein expressed on activated T cells, and some other cell types, such as granulocytes and cells of the monocyte/macropgage lineage.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human thymic clone B12

Clone: BB27

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD101 antibody (PE) (ABIN1981894)

Grassi, Dezutter-Dambuyant, McIlroy, Jacquet, Yoneda, Imamura, Boumsell, Schmitt, Autran, Debré, Hosmalin: "Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules." in: Journal of leukocyte biology, Vol. 64, Issue 4, pp. 484-93, (1998) (PubMed).

Bagot, Martinel, Charue, Weill, Boulland, Wechsler, Freeman, Bensussan, Boumsell: "CD101 is expressed by skin dendritic cells. Role in T-lymphocyte activation." in: Tissue antigens, Vol. 50, Issue 5, pp. 439-48, (1998) (PubMed).

Target Details for CD101

Target: CD101

Alternative Name: CD101 (CD101 Products)

Synonyms: Gm1016 antibody, Gm734 antibody, Igsf2 antibody, EWI-101 antibody, IGSF2 antibody, V7 antibody, CD101 molecule antibody, CD101 antigen antibody, CD101 antibody, Cd101 antibody

Background: CD101 Molecule,CD101 is a type I transmembrane glycoprotein, which forms disulfide-linked homodimers. It is expressed on activated T cells, as well as on granulocytes, monocytes, dendritic cells or mucosal T cells. It plays a major role in the activation of T cells by skin dendritic cells. Function of CD101 has not been fully elucidated, but in mice its knock-out results in liver autoimmune disease induced by Novosphingobium aromaticivorans.,IGSF2, V7, EWI-101

Gene ID: 9398

UniProt: Q93033