C5AR1 antibody (AA 15-27) (PE)

Catalog No. ABIN1981892

Product Details anti-C5AR1 Antibody (PE)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Binding Specificity: AA 15-27

Reactivity: Human, Cow, Rabbit, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5AR1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody S5/1 recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade.

Cross-Reactivity (Details): Human, Bovine, Ferret, Rabbit

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR

Clone: S5-1

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-C5AR1 antibody (PE) (ABIN1981892)

Camous, Roumenina, Bigot, Brachemi, Frémeaux-Bacchi, Lesavre, Halbwachs-Mecarelli: "Complement alternative pathway acts as a positive feedback amplification of neutrophil activation." in: Blood, Vol. 117, Issue 4, pp. 1340-9, (2011) (PubMed).

Conway Morris, Kefala, Wilkinson, Dhaliwal, Farrell, Walsh, Mackenzie, Reid, Davidson, Haslett, Rossi, Sallenave, Simpson: "C5a mediates peripheral blood neutrophil dysfunction in critically ill patients." in: American journal of respiratory and critical care medicine, Vol. 180, Issue 1, pp. 19-28, (2009) (PubMed).

Cain, Monk: "The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg(74)." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7165-9, (2002) (PubMed).

Kiener, Baghestanian, Dominkus, Walchshofer, Ghannadan, Willheim, Sillaber, Graninger, Smolen, Valent: "Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis." in: Arthritis and rheumatism, Vol. 41, Issue 2, pp. 233-45, (1998) (PubMed).

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: CD88 / C5aR (C5AR1 Products)

Synonyms: C5A antibody, C5AR antibody, C5R1 antibody, CD88 antibody, C5aR antibody, C5r1 antibody, Cd88 antibody, D7Msu1 antibody, GPR77 antibody, complement C5a receptor 1 antibody, complement component 5a receptor 1 antibody, C5AR1 antibody, C5ar1 antibody, c5ar1 antibody

Background: Complement C5a receptor 1,CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.,C5A, C5R1, C5AR1

Gene ID: 728

UniProt: P21730

Pathways: Complement System