CD235a/GYPA antibody (FITC)

Catalog No. ABIN1981887

Product Details anti-CD235a/GYPA Antibody (FITC)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

Cross-Reactivity (Details): Human, Rat

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Membrane preparation from splenic hairy cell leukemia

Clone: JC159

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD235a/GYPA antibody (FITC) (ABIN1981887)

Yamauchi, Takenaka, Urata, Shima, Kikushige, Tokuyama, Iwamoto, Nishihara, Iwasaki, Miyamoto, Honma, Nakao, Matozaki, Akashi: "Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment." in: Blood, Vol. 121, Issue 8, pp. 1316-25, (2013) (PubMed).

Alijotas-Reig, Palacio-Garcia, Llurba, Vilardell-Tarres: "Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review." in: Fertility and sterility, Vol. 99, Issue 2, pp. 441-9, (2013) (PubMed).

Maijenburg, Kleijer, Vermeul, Mul, van Alphen, van der Schoot, Voermans: "The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging." in: Haematologica, Vol. 97, Issue 2, pp. 179-83, (2012) (PubMed).

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a (GYPA Products)

Synonyms: GYPA antibody, gpa antibody, AI853584 antibody, CD235a antibody, GPA antibody, GPErik antibody, GPSAT antibody, HGpMiV antibody, HGpMiXI antibody, HGpSta(C) antibody, MN antibody, MNS antibody, PAS-2 antibody, glycophorin A (MNS blood group) antibody, glycophorin A antibody, GYPA antibody, Gypa antibody

Background: Glycophorin A (MNS blood group),CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group

Gene ID: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location