CD1c antibody

Catalog No. ABIN1981870

Product Details anti-CD1c Antibody

Target: CD1c (CD1C) (T-cell surface glycoprotein CD1c (CD1C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD1c antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: The mouse monoclonal antibody L161 recognizes an extracellular epitope of CD1c, (R7), a 43 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: human thymocytes

Clone: L161

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Do not use after expiration date stamped on vial label.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD1c antibody (ABIN1981870)

Scharf, Li, Hawk, Garzón, Zhang, Fox, Kazen, Shah, Haddadian, Gumperz, Saghatelian, Faraldo-Gómez, Meredith, Piccirilli, Adams: "The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation." in: Immunity, Vol. 33, Issue 6, pp. 853-62, (2010) (PubMed).

del C Salamone, Mendiguren, Salamone, Fainboim: "Membrane trafficking of CD1c on activated T cells." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 567-77, (2001) (PubMed).

Briken, Jackman, Watts, Rogers, Porcelli: "Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 281-8, (2000) (PubMed).

Todate, Chida, Suda, Imokawa, Sato, Ide, Tsuchiya, Inui, Nakamura, Asada, Hayakawa, Nakamura: "Increased numbers of dendritic cells in the bronchiolar tissues of diffuse panbronchiolitis." in: American journal of respiratory and critical care medicine, Vol. 162, Issue 1, pp. 148-53, (2000) (PubMed).

Target Details for CD1c

Target: CD1c (CD1C) (T-cell surface glycoprotein CD1c (CD1C))

Alternative Name: CD1c (CD1C Products)

Synonyms: BDCA1 antibody, CD1 antibody, CD1A antibody, R7 antibody, CD1c molecule antibody, CD1C antibody

Background: CD1c molecule,CD1c (also known as R7 or BDCA1) together with CD1a and b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with alpha/beta and gamma/delta T cells.,R7, BDCA1

Gene ID: 911

UniProt: P29017