HRAS antibody (AA 82-176)

Catalog No. ABIN1980538

Product Details anti-HRAS Antibody

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Binding Specificity: AA 82-176

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against HRAS. It has been selected for its ability to recognize HRAS in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: HRAS (Phe82-Glu176)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Handling Advice: Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) (HRAS Products)

Background: Alternative Names: HRAS1, K-Ras, N-Ras, RASH1, C-Bas/Has, H-Ras, Transforming protein p21

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling