MARCKS antibody (Ser158)
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- Target See all MARCKS Antibodies
- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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Binding Specificity
- Ser158
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MARCKS antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- This antibody detects endogenous levels of total MARCKS protein.
- Purification
- Immunoaffinity Chromatography using epitope-specific immunogen.
- Immunogen
- The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-Sp-F-K).
- Top Product
- Discover our top product MARCKS Primary Antibody
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-65) antibody
MARCKS Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 1-100) antibodyMARCKS Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibodyMARCKS Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer162) antibodyMARCKS Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer158) antibodyMARCKS Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (Internal Region) antibodyMARCKS Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer152), (Ser156) antibodyMARCKS Reactivity: Human, Mouse, Rat, Cow, Chicken, Xenopus laevis WB Host: Rabbit Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibodyMARCKS Reactivity: Human, Mouse WB, ELISA, IF, IHC, IHC (p) Host: Mouse Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 31-120) antibodyMARCKS Reactivity: Rat ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer159) antibodyMARCKS Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody (in aliquots) at -20 °C.
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- Target
- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
- Alternative Name
- MARCKS (MARCKS Products)
- Background
- MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
- Gene ID
- 4082
- NCBI Accession
- NP_002347
- UniProt
- P29966
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