Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Src antibody AP02687PU detects endogenous levels of total Src protein.
Purification
Immunoaffinity Chromatography.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-Yp-Q-P).
NCOA1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB14285
unconjugated
Application Notes
Western blot (1/500-1/1000). Immunohistochemistry on Paraffin Sections (1/50-1/100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at -20 °C.