CHEK1 antibody (Ser317)
-
- Target See all CHEK1 Antibodies
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
-
Binding Specificity
- Ser317
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This CHEK1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Chk1 antibody AP02645PU detects endogenous levels of total Chk1 protein.
- Purification
- Immunoaffinity chromatography.
- Immunogen
- The antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of serine 317 (S-S-Sp-Q-P)
- Top Product
- Discover our top product CHEK1 Primary Antibody
-
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibodyCHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer286) antibodyCHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibodyCHEK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibodyCHEK1 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibodyCHEK1 Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibodyCHEK1 Reactivity: Human WB, IHC (p), DB Host: Rabbit Polyclonal RB7845-RB18425 unconjugated
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 Reactivity: Human, Mouse WB, ELISA Host: Mouse Monoclonal 2G1D5 unconjugated
anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibodyCHEK1 Reactivity: Mouse, Chicken ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Checkpoint Kinase 1 (CHEK1) antibodyCHEK1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Monoclonal 2F2 unconjugated Recombinant Antibody
-
- Application Notes
-
Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody (in aliquots) at -20 °C.
-
- Target
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
- Alternative Name
- CHK1 (CHEK1 Products)
- Background
- One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
- Gene ID
- 1111
- NCBI Accession
- NP_001107593
- UniProt
- O14757
- Pathways
- p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair
-
