Bcl-2 antibody (Ser70)
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- Target See all Bcl-2 (BCL2) Antibodies
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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Binding Specificity
- Ser70
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Bcl-2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- BCL-2 antibody detects endogenous levels of total BCL-2 protein.
- Purification
- Affinity chromatography
- Immunogen
- Synthetic non-phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L)
- Top Product
- Discover our top product BCL2 Primary Antibody
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- Application Notes
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Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
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- Target
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Alternative Name
- Bcl-2 (BCL2 Products)
- Synonyms
- Bcl-2 antibody, PPP1R50 antibody, bcl-2 antibody, AW986256 antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, BCL-2 antibody, Bcl2 antibody, BCL2, apoptosis regulator antibody, B cell leukemia/lymphoma 2 antibody, B-cell CLL/lymphoma 2a antibody, B-cell CLL/lymphoma 2 protein antibody, BCL2 antibody, Bcl2 antibody, bcl2a antibody, BCL-2 antibody
- Background
- BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
- Gene ID
- 596
- NCBI Accession
- NP_000624
- UniProt
- P10415
- Pathways
- MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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