Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
STAT4 antibody detects endogenous levels of total STAT4 protein.
Purification
Affinity-chromatography using epitope-specific immunogen
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human STAT4 around the phosphorylation site of tyrosine 693 (K-G-Yp-V-P).
Western Blot: 1: 500-1: 1000. Immunohistochemistryon paraffin embedded tissue: 1: 50-1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Target
STAT4
(Signal Transducer and Activator of Transcription 4 (STAT4))