GSK3 alpha antibody (Ser21)

Catalog No. ABIN197104

Product Details anti-GSK3 alpha Antibody

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Binding Specificity: Ser21

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: GSK3alpha Antibody detects endogenous levels of total GSK3α protein.

Purification: Affinity chromatography

Immunogen: Synthetic non-phosphopeptide derived from human GSK3alpha around the phosphorylation site of serine 21 (T-S-SP-F-A).

Application Details

Application Notes: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3 alpha (GSK3a Products)

Synonyms: 2700086H06Rik antibody, GSK3A antibody, GSK-3[a] antibody, GSK-3[b] antibody, GSK3 antibody, gsk3a antibody, xgsk3 antibody, glycogen synthase kinase 3 alpha antibody, glycogen synthase kinase 3 alpha b antibody, glycogen synthase kinase 3 alpha S homeolog antibody, GSK3A antibody, Gsk3a antibody, gsk3a antibody, gsk3ab antibody, gsk3a.S antibody, Bm1_31380 antibody

Background: Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily, GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97 % sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

Gene ID: 2931

NCBI Accession: NP_063937

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect