MEF2A antibody detects endogenous levels of MEF2A only when phosphorylated at serine 408.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-SP-P-P).
Immunohistochemistry: 1: 50approx. 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MEF2A (myocyte specific enhancer factor 2) belongs to a family of DNA binding regulatory proteins. The MEF2 family of transcription factors is highly expressed in the brain when neurons undergo dendritic maturation and synapse formation. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. MEF2A also has key roles in cardiac and skeletal muscle development.Synonyms: MEF2, Myocyte-specific enhancer factor 2A, Serum response factor-like protein 1