The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. β-Catenin (phospho-Thr41/Ser45) antibody detects endogenous levels of β-Catenin only when phosphorylated at threonine 41/serine 45.
Purification
Immunoaffinity chromatography.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from humanbeta- Catenin around the phosphorylation site of threonine 41/serine 45 (A-T-TP-T-A-P-SP-L-S).
Suitable for use in Western blot (1: 500approx. 1: 1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.