NEFL antibody

Catalog No. ABIN1960129

Product Details anti-NEFL Antibody

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NF-L / NEFL

Purification: Purified

Immunogen: To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.

Type of Immunogen: Purified protein

Isotype: IgY

Application Details

Application Notes: Approved: ICC (1:5000), IF (1:5000), IHC, IHC-P (1:500), WB (1:10000)

Usage: The IgY solution can be used at dilutions of at least 1:5000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NF-L / NEFL (NEFL Products)

Synonyms: NEFL antibody, nefl antibody, zgc:136626 antibody, AI847934 antibody, CMT2E antibody, NF-L antibody, NF68 antibody, Nfl antibody, CMT1F antibody, NFL antibody, XNF-L antibody, neurofilament light antibody, neurofilament, light polypeptide antibody, neurofilament, light polypeptide b antibody, neurofilament, light L homeolog antibody, NEFL antibody, neflb antibody, Nefl antibody, nefl.L antibody

Background: Name/Gene ID: NEFL

Synonyms: NEFL, 68 kDa neurofilament protein, CMT1F, CMT2E, NF68, NF-L, NFL, Neurofilament subunit NF-L

Gene ID: 4747