RFP antibody

Catalog No. ABIN1952571

Product Details anti-RFP Antibody

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
ImmunogenType: Fusion protein

Clone: 8E5-G7

Isotype: IgG2a

Application Details

Application Notes: ELISA (1:20000 - 1:50000), WB (1:1000 - 1:3000),
Anti-RFP antibodies is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, . Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP / Red Fluorescent Protein (RFP Products)