BrdU antibody

Catalog No. ABIN1952323

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA

Specificity: Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with Bromodeoxyuridine-KLH followed by hybridoma development.

Type of Immunogen: Hapten

Clone: 29G6-E8

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), IF (1:500 - 1:3000), IHC (1:100 - 1:500), WB (1:2000 - 1:5000)

Usage: Anti-BrdU Antibody has been tested as suitable for immunoblot assays. Antibody may be suitable for additional immunoassays including immunofluorescence, flow cytometry and immunohistochemistry. Antibody will detect incorporated BrdU thymidine analog from replicated cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, sterile filtered

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU (BrdU Products)

Target Type: Chemical