ARHGEF1 antibody

Catalog No. ABIN1944955

Product Details anti-ARHGEF1 Antibody

Target: ARHGEF1 (rho Guanine Nucleotide Exchange Factor (GEF) 1 (ARHGEF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Isotype: IgG

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-ARHGEF1 antibody (ABIN1944955)

Hart, Jiang, Kozasa, Roscoe, Singer, Gilman, Sternweis, Bollag: "Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13." in: Science (New York, N.Y.), Vol. 280, Issue 5372, pp. 2112-4, (1998) (PubMed).

Aasheim, Pedeutour, Smeland: "Characterization, expression and chromosomal localization of a human gene homologous to the mouse Lsc oncogene, with strongest expression in hematopoetic tissues." in: Oncogene, Vol. 14, Issue 14, pp. 1747-52, (1997) (PubMed).

Hart, Sharma, elMasry, Qiu, McCabe, Polakis, Bollag: "Identification of a novel guanine nucleotide exchange factor for the Rho GTPase." in: The Journal of biological chemistry, Vol. 271, Issue 41, pp. 25452-8, (1996) (PubMed).

Target Details for ARHGEF1

Target: ARHGEF1 (rho Guanine Nucleotide Exchange Factor (GEF) 1 (ARHGEF1))

Alternative Name: ARHGEF1 (ARHGEF1 Products)

Synonyms: arhgef1 antibody, cb473 antibody, zgc:158261 antibody, ARHGEF1 antibody, GEF1 antibody, LBCL2 antibody, LSC antibody, P115-RHOGEF antibody, SUB1.5 antibody, Lbcl2 antibody, Lsc antibody, Rho guanine nucleotide exchange factor (GEF) 1a antibody, Rho guanine nucleotide exchange factor 1 antibody, Rho guanine nucleotide exchange factor (GEF) 1 antibody, arhgef1a antibody, ARHGEF1 antibody, arhgef1 antibody, Arhgef1 antibody

Background: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation.

Molecular Weight: 102435 Da

Gene ID: 9138

UniProt: Q92888

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling