Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage
4 °C,-20 °C
Goshima, Kawamura, Fukumoto, Miura, Honma, Satoh, Wakamatsu, Yamamoto, Kimura, Nishikawa, Andoh, Iida, Ishikawa, Ito, Kagawa, Kaminaga, Kanehori, Kawakami, Kenmochi, Kimura, Kobayashi, Kuroita et al.: "Human protein factory for converting the transcriptome into an in vitro-expressed proteome,. ..." in: Nature methods, Vol. 5, Issue 12, pp. 1011-7, (2008) (PubMed).
McCauliffe, Yang, Wilson, Sontheimer, Capra: "The 5'-flanking region of the human calreticulin gene shares homology with the human GRP78, GRP94, and protein disulfide isomerase promoters." in: The Journal of biological chemistry, Vol. 267, Issue 4, pp. 2557-62, (1992) (PubMed).
Rokeach, Haselby, Meilof, Smeenk, Unnasch, Greene, Hoch: "Characterization of the autoantigen calreticulin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 9, pp. 3031-9, (1991) (PubMed).
McCauliffe, Lux, Lieu, Sanz, Hanke, Newkirk, Bachinski, Itoh, Siciliano, Reichlin: "Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen." in: The Journal of clinical investigation, Vol. 85, Issue 5, pp. 1379-91, (1990) (PubMed).
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).