FEN1 antibody

Catalog No. ABIN1944939

Product Details anti-FEN1 Antibody

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Isotype: IgG

Application Details

Application Notes: IF: 1:100. WB: 1:1000. IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-FEN1 antibody (ABIN1944939)

Taylor, Noguchi, Totoki, Toyoda, Kuroki, Dewar, Lloyd, Itoh, Takeda, Kim, She, Barlow, Bloom, Bruford, Chang, Cuomo, Eichler, FitzGerald, Jaffe, LaButti, Nicol, Park, Seaman, Sougnez, Yang, Zimmer et al.: "Human chromosome 11 DNA sequence and analysis including novel gene identification. ..." in: Nature, Vol. 440, Issue 7083, pp. 497-500, (2006) (PubMed).

Shen, Nolan, Sklar, Park: "Essential amino acids for substrate binding and catalysis of human flap endonuclease 1." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9173-6, (1996) (PubMed).

Murray, Tavassoli, al-Harithy, Sheldrick, Lehmann, Carr, Watts: "Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4878-88, (1994) (PubMed).

Robins, Pappin, Wood, Lindahl: "Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coli DNA polymerase I." in: The Journal of biological chemistry, Vol. 269, Issue 46, pp. 28535-8, (1994) (PubMed).

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1 (FEN1 Products)

Synonyms: FEN-1 antibody, FEN1 antibody, AW538437 antibody, MF1 antibody, RAD2 antibody, cb879 antibody, fen-1 antibody, fen1 antibody, mf1 antibody, rad2 antibody, xFEN1a antibody, flap structure-specific endonuclease 1 antibody, flap endonuclease 1-like antibody, Flap endonuclease-1 antibody, 5'-3' exonuclease family protein antibody, flap structure specific endonuclease 1 antibody, flap structure-specific endonuclease 1 L homeolog antibody, nuclease, Rad2 family antibody, FEN1 antibody, LOC100808044 antibody, Bm1_49605 antibody, AT5G26680 antibody, Fen1 antibody, fen1 antibody, fen1.L antibody

Background: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Molecular Weight: 42593 Da

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA