This Phenylalanine Hydroxylase antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
B
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Immunogen
This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.
Reactivity: Human
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25. IHC-P: 1:25
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Siltberg-Liberles, Steen, Svebak, Martinez: "The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum." in: Gene, Vol. 427, Issue 1-2, pp. 86-92, (2008) (PubMed).
Miranda, Teigen, Thórólfsson, Svebak, Knappskog, Flatmark, Martínez: "Phosphorylation and mutations of Ser(16) in human phenylalanine hydroxylase. Kinetic and structural effects." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40937-43, (2002) (PubMed).
Cotton, McAdam, Jennings, Morgan: "A monoclonal antibody to aromatic amino acid hydroxylases. Identification of the epitope." in: The Biochemical journal, Vol. 255, Issue 1, pp. 193-6, (1989) (PubMed).
Kwok, Ledley, DiLella, Robson, Woo: "Nucleotide sequence of a full-length complementary DNA clone and amino acid sequence of human phenylalanine hydroxylase." in: Biochemistry, Vol. 24, Issue 3, pp. 556-61, (1985) (PubMed).