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Hsc70 antibody (C-Term)

Name: Hsc70 antibody (C-Term)
SKU: ABIN1944844
Price: 451 USD
Availability: InStock

HSPA8 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16528 unconjugated

Western blot analysis of lysates from rat H-4-II-E, mouse NIH/3T3 cell line (from left to right), using HS Antibody (C-term) (ABIN1944844 and ABIN2839489). (Hsc70 antibody (C-Term))

HS Antibody (C-term) (ABIN1944844 and ABIN2839489) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Hsc70 antibody (C-Term))

IHC (p)

Confocal immunofluorescent analysis of HS Antibody (C-term) (ABIN1944844 and ABIN2839489) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Hsc70 antibody (C-Term))

HS Antibody (C-term) (ABIN1944844 and ABIN2839489) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (Hsc70 antibody (C-Term))

FACS

4 images

(2 references)

Catalog No. ABIN1944844

Datasheet as PDF

Target See all Hsc70 (HSPA8) Antibodies

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Binding Specificity
14

7

6

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 539-569, C-Term
Reactivity
83

48

44

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Human
Host
71

28

7

2
Rabbit
Compatible Secondaries
Clonality
68

39
Polyclonal
Conjugate
60

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
Application
94

51

41

30

29

28

15

13

11

11

11

5

4

2

2

2

2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Predicted Reactivity

B, Ha, Hs, M, Rat

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539-569 amino acids from the C-terminal region of human HSPA8.

Clone

RB16528

Isotype

Ig Fraction

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anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA, IP, FACS Host: Mouse Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 82-110), (N-Term) antibody
HSPA8 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB17655 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA Host: Mouse Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse WB, ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 Reactivity: Human, Rat, Mouse, Cow, Sheep, Pig, Zebrafish (Danio rerio), Goat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Expiry Date

6 months
Tsukahara, Yoshioka, Muraki: "Molecular and functional characterization of HSC54, a novel variant of human heat-shock cognate protein 70." in: Molecular pharmacology, Vol. 58, Issue 6, pp. 1257-63, (2000) (PubMed).

Egerton, Moritz, Druker, Kelso, Simpson: "Identification of the 70kD heat shock cognate protein (Hsc70) and alpha-actinin-1 as novel phosphotyrosine-containing proteins in T lymphocytes." in: Biochemical and biophysical research communications, Vol. 224, Issue 3, pp. 666-74, (1996) (PubMed).
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name

HSPA8 (HSPA8 Products)

Background

HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.

Molecular Weight

70898

Gene ID

3312

NCBI Accession

NP_006588, NP_694881

UniProt

P11142