FAS
Reactivity: Human
WB, ELISA, IP
Host: Mouse
Monoclonal
1B6
unconjugated
Application Notes
WB: 1:1000. IHC: 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage
4 °C,-20 °C
Cascino, Papoff, De Maria, Testi, Ruberti: "Fas/Apo-1 (CD95) receptor lacking the intracytoplasmic signaling domain protects tumor cells from Fas-mediated apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 1, pp. 13-7, (1996) (PubMed).
Liu, Cheng, Mountz: "Differential expression of human Fas mRNA species upon peripheral blood mononuclear cell activation." in: The Biochemical journal, Vol. 310 ( Pt 3), pp. 957-63, (1995) (PubMed).
Cascino, Fiucci, Papoff, Ruberti: "Three functional soluble forms of the human apoptosis-inducing Fas molecule are produced by alternative splicing." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 6, pp. 2706-13, (1995) (PubMed).
Oehm, Behrmann, Falk, Pawlita, Maier, Klas, Li-Weber, Richards, Dhein, Trauth: "Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen." in: The Journal of biological chemistry, Vol. 267, Issue 15, pp. 10709-15, (1992) (PubMed).
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).