CCL20 antibody (APC)

Catalog No. ABIN192399

Product Details anti-CCL20 Antibody (APC)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL20 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The antibody UCHL1 recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human IL-2 dependent T cells

Clone: UCHL1

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CCL20 antibody (APC) (ABIN192399)

Ricciardi, Manfrini, Alfieri, Calamita, Crosti, Gallo, Müller, Pagani, Abrignani, Biffo: "The Translational Machinery of Human CD4+ T Cells Is Poised for Activation and Controls the Switch from Quiescence to Metabolic Remodeling." in: Cell metabolism, Vol. 28, Issue 6, pp. 895-906.e5, (2019) (PubMed).

Akbar, Terry, Timms, Beverley, Janossy: "Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 7, pp. 2171-8, (1988) (PubMed).

Beverley, Merkenschlager, Terry: "Phenotypic diversity of the CD45 antigen and its relationship to function." in: Immunology. Supplement, Vol. 1, pp. 3-5, (1988) (PubMed).

Terry, Brown, Beverley: "The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45." in: Immunology, Vol. 64, Issue 2, pp. 331-6, (1988) (PubMed).

Beverley: "Human T cell subsets." in: Immunology letters, Vol. 14, Issue 4, pp. 263-7, (1987) (PubMed).

Norton, Ramsay, Smith, Beverley, Isaacson: "Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues." in: Journal of clinical pathology, Vol. 39, Issue 4, pp. 399-405, (1986) (PubMed).

Smith, Brown, Rowe, Callard, Beverley: "Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1." in: Immunology, Vol. 58, Issue 1, pp. 63-70, (1986) (PubMed).

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CD45R0 (CCL20 Products)

Synonyms: MIP-3ALPHA antibody, CCL20 antibody, si:dkey-150o13.1 antibody, CKb4 antibody, LARC antibody, MIP-3-alpha antibody, MIP-3a antibody, MIP3A antibody, SCYA20 antibody, ST38 antibody, MIP-3A antibody, MIP-3[a] antibody, Scya20 antibody, exodus-1 antibody, C-C motif chemokine ligand 20 antibody, chemokine (C-C motif) ligand 20b antibody, chemokine (C-C motif) ligand 20 antibody, CCL20 antibody, ccl20b antibody, Ccl20 antibody

Background: CD45R0 is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.,PTPRCR, T200R0

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection