STIM1 antibody (C-Term)

Catalog No. ABIN192394

Product Details anti-STIM1 Antibody

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Cross-Reactivity (Details): Human, Mouse, Rat

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Synthetized peptide (C-terminal cytoplasmic part of STIM1).

Clone: CDN3H4

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry: Methanol-aceton fixation, positive control: HeLa human cervix carcinoma cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL.
Western blotting: Recommended dilution: 1 μg/mL, positive control: RBL rat basophilic leukemia cell line, both reducing and non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-STIM1 antibody (ABIN192394)

Hájková, Bugajev, Dráberová, Vinopal, Dráberová, Janá?ek, Dráber, Dráber: "STIM1-directed reorganization of microtubules in activated mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 913-23, (2011) (PubMed).

Ng, Ramduny, Airey, Singer, Keller, Shen, Tian, Valencik, Hume: "Orai1 interacts with STIM1 and mediates capacitative Ca2+ entry in mouse pulmonary arterial smooth muscle cells." in: American journal of physiology. Cell physiology, Vol. 299, Issue 5, pp. C1079-90, (2010) (PubMed).

Target Details for STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1 (STIM1 Products)

Synonyms: stim1 antibody, MGC76045 antibody, STIM1 antibody, si:dkey-24p1.5 antibody, MGC154748 antibody, si:ch211-112l17.2 antibody, Stim1 antibody, D11S4896E antibody, GOK antibody, TAM antibody, SIM antibody, stromal interaction molecule 1 antibody, stromal interaction molecule 1a antibody, stromal interaction molecule 1 L homeolog antibody, stromal interaction molecule 1b antibody, STIM1 antibody, stim1 antibody, stim1a antibody, stim1.L antibody, stim1b antibody, Stim1 antibody

Background: Stromal interaction molecule 1,STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.,GOK, TAM1, STRMK

Gene ID: 6786

UniProt: G0XQ39

Pathways: TCR Signaling, BCR Signaling