CD177 antibody (APC)

Catalog No. ABIN192245

Product Details anti-CD177 Antibody (APC)

Target: CD177 (CD177 Molecule (CD177))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD177 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.

Cross-Reactivity (Details): Non-Human Primates, Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human granulocytes

Clone: MEM-166

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177 (CD177 Products)

Synonyms: HNA-2a antibody, HNA2A antibody, NB1 antibody, NB1 GP antibody, PRV-1 antibody, PRV1 antibody, RGD1562941 antibody, CD177 antibody, MGC157159 antibody, 1190003K14Rik antibody, Pdp3 antibody, CD177 molecule antibody, CD177 antigen antibody, CD177 antibody, Cd177 antibody

Background: CD177 Molecule,CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).,NB1, PRV1, HNA2A

Gene ID: 57126

UniProt: Q8N6Q3