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HLA-DP/DR antibody (APC)

HLA-DP/DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-136 APC
Catalog No. ABIN192207
  • Target See all HLA-DP/DR products
    HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
    Reactivity
    • 16
    • 5
    Human
    Host
    • 16
    Mouse
    Clonality
    • 16
    Monoclonal
    Conjugate
    • 5
    • 4
    • 4
    • 2
    • 1
    This HLA-DP/DR antibody is conjugated to APC
    Application
    • 16
    • 8
    • 8
    • 5
    • 3
    • 1
    Flow Cytometry (FACS)
    Specificity
    The antibody MEM-136 recognizes a common extracellular epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    PHA-activated peripheral blood lymphocytes.
    Clone
    MEM-136
    Isotype
    IgG1
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

  • Target
    HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))
    Alternative Name
    HLA-DR+DP (HLA-DP/DR Products)
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