pan Keratin antibody (PE)

Catalog No. ABIN192048

Product Details anti-pan Keratin Antibody (PE)

Target: pan Keratin (panKRT)

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Cross-Reactivity (Details): Mammalian

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Clone: C-11

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-pan Keratin antibody (PE) (ABIN192048)

Petry, Kippenberger, Meissner, Kleemann, Kaufmann, Rieger, Wellenbrock, Reichenbach, Zöller, Valesky: "Directing adipose-derived stem cells into keratinocyte-like cells: impact of medium composition and culture condition." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 32, Issue 11, pp. 2010-2019, (2019) (PubMed).

Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." in: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." in: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Cytokeratin (Pan-reactive) (panKRT Products)

Synonyms: xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody

Background: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT