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OSBPL5 antibody (N-Term)

Verified OSBPL5 Reactivity: Human, Rat WB, ELISA, IF, IHC, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN190918
  • Target See all OSBPL5 Antibodies
    OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))
    Binding Specificity
    • 1
    • 1
    N-Term
    Reactivity
    Human, Rat
    Host
    • 2
    • 2
    Goat
    Clonality
    • 4
    Polyclonal
    Conjugate
    • 4
    This OSBPL5 antibody is un-conjugated
    Application
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Purpose
    ORP5 (OBPH1)
    Sequence
    KEEAFLRRRF SLC
    Specificity
    This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1, NP_001137535.1) encode the same isoform.
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1, NP_663613.1, NP_001137535.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 μg/mL.

    Western Blot: Approx. 100 kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4 kDa according to Human NP_065947.1 and 101.7 kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3 μg/mL. Primary

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))
    Alternative Name
    OSBPL5 (OSBPL5 Products)
    Synonyms
    MGC109308 antibody, OSBPL5 antibody, OBPH1 antibody, ORP5 antibody, 1110006M06Rik antibody, AI462538 antibody, Obph1 antibody, Osbp2 antibody, oxysterol binding protein-like 5 antibody, oxysterol binding protein like 5 antibody, Osbpl5 antibody, OSBPL5 antibody, osbpl5 antibody
    Background
    OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5
    Gene ID
    114879, 79196
    NCBI Accession
    NP_065947, NP_663613, NP_001137535
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