OSBPL5 antibody (N-Term)
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- Target See all OSBPL5 Antibodies
- OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))
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Binding Specificity
- N-Term
- Reactivity
- Human, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This OSBPL5 antibody is un-conjugated
- Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
- Purpose
- ORP5 (OBPH1)
- Sequence
- KEEAFLRRRF SLC
- Specificity
- This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1, NP_001137535.1) encode the same isoform.
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1, NP_663613.1, NP_001137535.1.
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 μg/mL.
Western Blot: Approx. 100 kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4 kDa according to Human NP_065947.1 and 101.7 kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3 μg/mL. Primary
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))
- Alternative Name
- OSBPL5 (OSBPL5 Products)
- Synonyms
- MGC109308 antibody, OSBPL5 antibody, OBPH1 antibody, ORP5 antibody, 1110006M06Rik antibody, AI462538 antibody, Obph1 antibody, Osbp2 antibody, oxysterol binding protein-like 5 antibody, oxysterol binding protein like 5 antibody, Osbpl5 antibody, OSBPL5 antibody, osbpl5 antibody
- Background
- OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5
- Gene ID
- 114879, 79196
- NCBI Accession
- NP_065947, NP_663613, NP_001137535
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