Iba1 antibody (C-Term)
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- Target See all Iba1 (IBA1) Antibodies
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Iba1 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- AIF1 / IBA1 (isoforms 1 + 3)
- Sequence
- YEEKAREKEK P
- Specificity
- This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-YEEKAREKEKP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3.
- Isotype
- IgG
- Top Product
- Discover our top product IBA1 Primary Antibody
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10μg/ - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
- Alternative Name
- AIF1 (IBA1 Products)
- Synonyms
- AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody
- Background
- AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
- Gene ID
- 199
- NCBI Accession
- NP_116573, NP_001614
- Pathways
- Smooth Muscle Cell Migration
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