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Iba1 antibody (C-Term)

Verified IBA1 Reactivity: Human IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN190906
  • Target See all Iba1 (IBA1) Antibodies
    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
    Binding Specificity
    • 16
    • 16
    • 15
    • 9
    • 8
    • 8
    • 7
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 94
    • 58
    • 53
    • 1
    • 1
    Human
    Host
    • 83
    • 32
    • 4
    Goat
    Clonality
    • 86
    • 33
    Polyclonal
    Conjugate
    • 49
    • 17
    • 13
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Iba1 antibody is un-conjugated
    Application
    • 72
    • 43
    • 38
    • 21
    • 17
    • 14
    • 14
    • 11
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    AIF1 / IBA1 (isoforms 1 + 3)
    Sequence
    YEEKAREKEK P
    Specificity
    This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-YEEKAREKEKP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3.
    Isotype
    IgG
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    Discover our top product IBA1 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10μg/

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
    Alternative Name
    AIF1 (IBA1 Products)
    Synonyms
    AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody
    Background
    AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
    Gene ID
    199
    NCBI Accession
    NP_116573, NP_001614
    Pathways
    Smooth Muscle Cell Migration
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