Neuroligin 2 antibody (Internal Region)

Catalog No. ABIN190848

Product Details anti-Neuroligin 2 Antibody

Target: Neuroligin 2 (NLGN2)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neuroligin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Neuroligin 2

Sequence: NPPDTDIRDP GKK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NPPDTDIRDPGKK, from the internal region of the protein sequence according to NP_065846.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in Human Pancrease lysates (calculated MW of 90.8 kDa according to NP_065846.1). Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentrati

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Neuroligin 2 antibody (ABIN190848)

Zelano, Berg, Thams, Hailer, Cullheim: "SynCAM1 expression correlates with restoration of central synapses on spinal motoneurons after two different models of peripheral nerve injury." in: The Journal of comparative neurology, Vol. 517, Issue 5, pp. 670-82, (2009) (PubMed).

Target Details for Neuroligin 2

Target: Neuroligin 2 (NLGN2)

Alternative Name: NLGN2 (NLGN2 Products)

Synonyms: Nlgn2 antibody, GB10066 antibody, NLGN2 antibody, CCE-L2 antibody, NV50031 antibody, Nv123771 antibody, NL2 antibody, RP23-422L16.24 antibody, neuroligin 2 antibody, LOC410779 antibody, NLGN2 antibody, LOC663865 antibody, LOC100123771 antibody, Nlgn2 antibody

Background: Neuroligin 2 , KIAA1366, NLGN2

Gene ID: 57555, 216856, 117096

NCBI Accession: NP_065846

Pathways: Cell-Cell Junction Organization, Synaptic Membrane