P4HA1 antibody (Internal Region)

Catalog No. ABIN190847

Product Details anti-P4HA1 Antibody

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This P4HA1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: P4HA1

Sequence: DFARKDEPDA FKE

Specificity: This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DFARKDEPDAFKE, from the internal region of the protein sequence according to NP_000908.2, NP_001017962.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 7 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for P4HA1

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Alternative Name: P4HA1 (P4HA1 Products)

Synonyms: P4HA antibody, AL022634 antibody, P4ha antibody, PHalphaI antibody, prolyl 4-hydroxylase subunit alpha-1 antibody, prolyl 4-hydroxylase subunit alpha 1 antibody, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide antibody, CpipJ_CPIJ000975 antibody, CpipJ_CPIJ017131 antibody, Tsp_03346 antibody, Tsp_03347 antibody, Tsp_15957 antibody, P4HA1 antibody, P4ha1 antibody

Background: P4HA1, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I, 4-PH alpha-1, P4HA , prolyl 4-hydroxylase, alpha I subunit

Gene ID: 5033, 18451, 64475

NCBI Accession: NP_000908, NP_001017962