SORBS1 antibody (Internal Region)

Catalog No. ABIN190836

Product Details anti-SORBS1 Antibody

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SORBS1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: SORBS1/ponsin (internal region)

Sequence: SERNDWEPPD KKVD

Specificity: This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1)

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SERNDWEPPDKKVD, from the internal region of the protein sequence according to NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1, NP_001030129.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas and Liver. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 65 kDa band in Human Adipose lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SORBS1

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Alternative Name: SORBS1 (SORBS1 Products)

Background: SORBS1, sorbin and SH3 domain containing 1, CAP, DKFZp451C066, DKFZp586P1422, FLAF2, FLJ12406, KIAA1296, R85FL, SH3D5, SH3P12, SORB1 , Fas-ligand associated factor 2, SH3-domain protein 5, SH3-domain protein 5 (ponsin), c-Cbl associated protein, ponsin

Gene ID: 10580, 20411

NCBI Accession: NP_006425, NP_056200, NP_001030126, NP_001030127, NP_001030128, NP_079267, NP_001030129

Pathways: Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process